链脲佐菌素, 2 型糖尿病, 糖尿病肾病, 动物模型

Objective To establish a diabetic rat model induced by streptozotocin and evaluate its renalinjury. Method

Four-week-old male Sprague-Dawley ( SD) rats were fed a high-fat diet and injected intraperitoneally with streptozotocin ( STZ) at a dose of 30 mg / kg to induce the diabetic model. During the experiment, the general condition of the rats was observed, and fasting blood glucose ( FBG) and body mass was measured. A FBG level ≥ 11. 1 mmol / L was used as the standard for establishing the type 2 diabetes model. After successful modeling, the following parameters were assessed: 24-hour urine protein ( UP ) , urine creatinine ( UCr) , serum creatinine ( SCr ) , and blood urea nitrogen ( BUN) content. Renal tissue was collected to calculate the kidney index (KI) , and HE staining, periodic acid-Schiff ( PAS) staining, and Masson staining were performed to observe renal injury. Result

Compared with the normal group, the model group showed decreased body weight and urine creatinine levels, while 24-hour urine protein, serum creatinine, BUN content, and kidney index were significantly increased ( all P<0. 05) . No obvious abnormalities were observed in the renal pathological staining. Conclusion SD rat model of type 2 diabetes can be established through a 13-week high-fat diet and STZ injection (30 mg / kg) . This model causes renal injury; however, no pathological changes such as glomerulosclerosis, mesangial cell proliferation, or renal fibrosis were observed under light microscopy.

streptozotocin, type 2 diabetes, diabetic kidney disease, animal models